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Cuando vives solo de recuerdos y no de realidades. Cuando no estamos incluidos en los planes futuros de esa otra persona.

Cell viability was measured by ATP production level using CellTiter-Glo luminescent cell viability assay kit according the manufacturer's instructions (Promega Corp Madison, WI). Cytotoxicity was determined by the amount of lactate dehydrogenase (LDH) activity released in the cell culture media using CytoTox96 Non-Radioactive Cytotoxicity Assay according the manufacturer's instructions (Promega Corp). Mitochondria preparations and ATP measurements. Mitochondria isolation and ATP measurements were performed using the mitochondria isolation kit and an Adenosine 5'-triphosphate (ATP) Bioluminescent Assay kits respectively according to the manufacturer's instructions (Sigma). Spinal cords from WT and Tg SOD1-G93A animals, 8 weeks of age, were dissected on ice and the tissue was quickly grinded in buffer A with a 3 ml Teflon pestle with 10 -15 strokes.

Chen CLiu YLu Cet al. Cancer-associated IDH2 mutants drive an acute myeloid leukemia that is susceptible to Brd4 inhibition. Genes Dev 2013;27:1974-1985.]